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Image Search Results
Journal: Materials Today Bio
Article Title: Enhanced inhibition of neuronal ferroptosis and regulation of microglial polarization with multifunctional traditional Chinese medicine active ingredients-based selenium nanoparticles for treating spinal cord injury
doi: 10.1016/j.mtbio.2025.101758
Figure Lengend Snippet: TSIIA/TMP/APS@Se NPs regulate the polarization of BV2 cells and improve the inflammatory microenvironment to rescue PC12 cells in a co-culture system. (A–D) The ELISA quantitative analysis of the inflammatory cytokines in BV2 cells after different treatments. (E) Schematic illustration of the modulation of TSIIA/TMP/APS@Se NPs on BV2 polarization. (F) Protein expression images of iNOS and Arg-1 in BV2 cells after different treatment of nanoparticles. (G) Flow cytometry result of the BV2 cells to identify the M1 phenotype (F4/80, CD16/32 double positive) and M2 phenotype (F4/80, CD206 double positive) after incubation with F4/80, CD16/32, CD206. (H) Schematic illustration of a co-cultured system between PC12 cells and BV2 cells, indicating outcomes of diverse treatments with Se NPs. (I) The representative images of Live/Dead stains of PC12 cells after different treatments of the co-cultured system. Scale bar = 200 μm ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, and ns ( P > 0.05) suggested no statistical difference.
Article Snippet: The immortalized
Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Incubation, Cell Culture
Journal: Frontiers in Cellular Neuroscience
Article Title: Microglia Express Insulin-Like Growth Factor-1 in the Hippocampus of Aged APP swe /PS1 ΔE9 Transgenic Mice
doi: 10.3389/fncel.2019.00308
Figure Lengend Snippet: IGF-1 mRNA is unchanged in BV2 cells and primary microglia after Aβ 42 exposure. The BV2 cell line and primary microglia from newborn mice were stimulated 24 h with 1 μM Aβ 42 whereafter, RNA was isolated, reverse transcribed and used for qPCR for IGF-1 mRNA (A,C) and CD11b mRNA (B,D) . Levels of IGF-1 mRNA (A,C) and CD11b mRNA (B,D) were unchanged after Aβ 42 exposure. Each dot represents 1 experiment. Bars indicate the medians and the error bars the 25 and 75% quartiles.
Article Snippet: The
Techniques: Isolation, Reverse Transcription